A simple, sensitive, rapid and precise RP-HPLC method has been developed for the estimation of Gemifloxacin in bulk and pharmaceutical formulations. Stainless steel column packed with C18 chemically bonded porous silica. The particle size of 5?m, length 4.6mm x 250 mm was used for the separation. The mobile phase consisting of Acetonitrile and Potassium dihydrogen phosphate (20:80), adjust the pH 3.0 by ortho phosphoric acid. The mobile phase was pumped at a flow rate of 1.0 ml/min and the detection was carried out at 266.0 nm. The retention time was found to be 11.166 min. This method is validated for Linearity, Specificity, Accuracy, System suitability, Precision, Ruggedness, and Robustness. The proposed method is a good approach for obtaining reliable results and found to be suitable for the routine analysis in Gemifloxacin in pharmaceutical formulations.
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